Binding of enzyme--IgG complexes in human serum to Protein-A Sepharose CL-4B.
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چکیده
منابع مشابه
Affinity purification of cellular retinoic acid-binding protein on 14-carboxy-13-cis-retinamide-sepharose 4B.
A biologically active bifunctional retinoid, ethyl 14-carboxyretinoate, has been synthesized and shown to bind cellular retinoic acid (RA)-binding protein (CRABP) via its free carboxy group. We describe herein the synthesis of 14-carboxy-13-cis-retinamide-Sepharose 4B, which is an affinity matrix bearing an all-trans-RA moiety, and thus was used to purify and characterize CRABP from chick-embry...
متن کاملCoupling of glycosaminoglycans to agarose beads (sepharose 4B).
1. Heparin, heparan sulphate, chondroitin sulphate and dermatan sulphate were covalently attached to beads of agarose activated by cyanogen bromide. The bond is probably mediated by the amino group of a serine or peptide residue at the reducing end of the polysaccharide chain. 2. The uptake of glycosaminoglycan during the coupling procedure is about 0.9mg/ml of wet gel. However, direct analysis...
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Human serum albumin (HSA) is an important protein that carries variety of substances like some hormones and drugs in blood. Pharmacological studies of the interaction of many drugs and HSA are reported during several decades, specially recently years. Interaction of cortisol and fluoxetine hydrochloride (FLX) (as a common anti-stress drug) with HSA (as their carrier in blood) has been studied s...
متن کاملFluoxetin Competes with Cortisol for Binding to Human Serum Albumin
Human serum albumin (HSA) is an important protein that carries variety of substances like some hormones and drugs in blood. Pharmacological studies of the interaction of many drugs and HSA are reported during several decades, specially recently years. Interaction of cortisol and fluoxetine hydrochloride (FLX) (as a common anti-stress drug) with HSA (as their carrier in blood) has been studied s...
متن کاملIdentification of Amino Acids Involve in Indium Binding To Serum Human Apo-Transferrin
Indium is a heavy metal belonging to group IIIa. It is used as a radioimaging and chemotherapeutic agent in diagnosis and also in the treatment of cancers. It is believed that indium may interfere with iron metabolism and reduce cell growth in cancer tissue. The present report was established to study the binding of iron and indium to apo-transferrin (apo-tf) and to identify amino acids involv...
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ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 1980
ISSN: 0009-9147,1530-8561
DOI: 10.1093/clinchem/26.2.297